Nitric oxide production in cultured endothelial cells from control and S. mansoni-infected mice.
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Nitric oxide (NO) production by confluent mesenteric endothelial cells in response to 100 µM ATP and 2 µM A23187 was measured in living cells using the fluorescent probe DAF-FM (2.5 µM) and a microplate fluorometer. Control mice: white bars (open, horizontal and vertical lines). Infected mice: gray bars (open, horizontal and vertical lines). Data are expressed as the mean and S.E.M. of 6–7 experiments performed in triplicate obtained from four different cultures and from different animals (ATP condition) or four replicates of a typical experiment (A23187 condition). Basal NO production observed in the absence of ATP or A23187 was considered as 100%. *P<0.05 vs. control mice; **P<0.05 vs. A23187 treatment in control mice, One-way ANOVA followed by Bonferroni's Multiple Comparison test.