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Neutrophil accumulation and myeloperoxidase (MPO) activity in the lungs after hemorrhagic shock and resuscitation (HSR).

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posted on 07.05.2013, 02:25 by Junko Kosaka, Hiroshi Morimatsu, Toru Takahashi, Hiroko Shimizu, Susumu Kawanishi, Emiko Omori, Yasumasa Endo, Naofumi Tamaki, Manabu Morita, Kiyoshi Morita

Lungs from the HSR group rats treated with or without biliverdin (BV) were excised 12 h after resuscitation. Neutrophils were stained by the naphthol AS-D chloroacetate method and MPO activity was measured. (A) Each photograph is representative of five independent experiments. The arrows indicate positively stained neutrophils (original magnification ×400, scale bar  = 50 µm). (B) The number of neutrophils in five nonconsecutive lung sections per rat at a magnification of ×400 (n = 5 per group). (C) Lung MPO activity (n = 6 per group). Data are presented as means ± standard deviation and were statistically evaluated using analysis of variance followed by Tukey–Kramer honestly significant difference test. *p<0.05 vs. vehicle/sham; p<0.05 vs. BV/sham; #p<0.05 vs. vehicle/HSR. Vehicle/sham, vehicle-administered animals subjected to sham surgery; BV/sham, BV-administered animals subjected to sham surgery; vehicle/HSR, vehicle-administered animals subjected to HSR; BV/HSR, BV-administered animals subjected to HSR.