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Neuroprotection of primary rat cortical neurons from a variety of insults.

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posted on 21.02.2013, 02:08 authored by Belen Tizon, Susmita Sahoo, Haung Yu, Sebastien Gauthier, Asok R. Kumar, Panaiyur Mohan, Matthew Figliola, Monika Pawlik, Anders Grubb, Yasuo Uchiyama, Urmi Bandyopadhyay, Ana Maria Cuervo, Ralph A. Nixon, Efrat Levy

Primary rat cortical neurons were cultured in B27-supplemented neurobasal medium in the presence of 10 µM H2O2 (A), 0.5 µM colchicine (B), or 0.1 µM staurosporine (C) and different concentrations of human CysC for 24 hours. Cell survival analyzed by the MTS assay is expressed as percentage of live cells in cultures incubated in B27-supplemented medium without CysC. Data are the mean and SEM of 3 experiments. F and P values determined by one way ANOVA for (A) were 7.45 and 0.01. Differences between CysC treated and untreated samples were calculated by Student's t-test for B and C.

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