NanoString analysis of expression changes for selected genes in response to IL4- and IL10-treatment.
Treating rat primary microglia for 6 hr with 20 ng/ml IL4 or 20 ng/ml IL10 resulted in gene expression changes that were specific for IL4 (A), specific for IL10 (B), or common to both treatments (C). mRNA expression was normalized to the housekeeping gene, HPRT1, and shown as mean ± SEM with the number of individual cultures indicated on each bar. [Note the differing Y-axis scales.] One-way ANOVA with Tukey's post-hoc test revealed differences from unstimulated (control) microglia: *p<0.05, **p<0.01, ***p<0.001.