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N. parisii spores exit apically out of intestinal cells and are not labeled with a C. elegans membrane marker.

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posted on 2011-09-15, 01:41 authored by Kathleen A. Estes, Suzannah C. Szumowski, Emily R. Troemel

(A) TEM indicating N. parisii spores inside intestinal cells and the intestinal lumen. Intestinal cells are false colored yellow (labeled I), the intestinal lumen and microvilli are false colored red (labeled L), the gonad is false colored green (labeled G) and body wall muscle is false colored purple (labeled M). Examples of intracellular spores in the intestinal cells are indicated with red upward arrows and an example of a lumenal extracellular spore is indicated with red rightward arrowhead. Spores were only observed inside intestinal cells and in the intestinal lumen, suggesting that they only exit apically from intestinal cells (not basolaterally). Scale bar is 10 µm. (B) N. parisii infected animal fixed and stained with Calcofluor White to label N. parisii spores inside intestinal cells. Scale bar is 20 µm. (C) N. parisii spores in the intestinal lumen of PGP-1::GFP animals are free of GFP, suggesting that they did not take C. elegans membrane upon exit into the lumen. Two spores in lumen are indicated with arrowheads. PGP-1::GFP labels the apical (lumenal) side of two intestinal cells. Scale bar is 5 µm.

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