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N-cadherin deficient cells are non-migratory in 3D Matrigel.

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posted on 24.01.2013, 00:41 by Yuanyuan Cui, Soichiro Yamada

(A) Immunoblots for N-cadherin, E-cadherin and cadherin 11 in N-cadherin knockdown cells and cells transfected with scrambled sequences. Tubulin is shown as a loading control. KD1, KD2 are two knockdown clones. Scr1, Scr2 are two clones transfected with corresponding scrambled sequences. (B) Immunostaining of N-cadherin in control cells (top panel) and knockdown cells (bottom panel). (C) Cell aggregate formation of knockdown cells and control cells over 3 hours in suspension. At hour 0, 1, 2, 3 in suspension, the numbers of cell aggregates analyzed were 144, 101, 133, 62 for parental PC3 cells, 147, 117, 107, 81 for KD1 cells, 142, 121, 107, 73 for KD2 cells, 146, 115, 87, 88 for Scr1 cells, 131, 147, 107, 68 for Scr2 cells, respectively. (D) 3D cell migration analyses of parental (N = 17), scramble shRNA (N = 17 for Scr1, N = 20 for Scr2) and knockdown cells (N = 37 for KD1, N = 33 for KD2). Statistical analysis of average speed is shown (**, P<0.01). All error bars are standard error of the mean. All scale bars are 20 μm.

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