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NMDA stimulation causes reversible retraction of oligodendrocyte processes.

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posted on 07.06.2013 by Manabu Makinodan, Aya Okuda-Yamamoto, Daisuke Ikawa, Michihiro Toritsuka, Tomohiko Takeda, Sohei Kimoto, Kouko Tatsumi, Hiroaki Okuda, Yu Nakamura, Akio Wanaka, Toshifumi Kishimoto

An untreated oligodendrocyte was stimulated with NMDA, which gradually caused retraction of the processes over 36 h, but the soma appeared intact. The leading edges of representative processes are marked by dots (A). The processes were progressively regenerated within 8 h after the removal of NMDA from the culture medium (B). The 8-h image of cell No. 1 viewed by immunofluorescence microscopy: MBP, green; DAPI, blue (C). Images of other representative cells (No. 2, No. 3), with NMDA and after its removal (D). Statistical analysis indicates that moderate NMDA treatment for 36 h significantly retract oligodendrocyte processes, which recovered 8 h after the removal of NMDA (E) (n = 5, each; ** p<0.01). ‘No.’ is an arbitrary reference number for individual oligodendrocytes.