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NF-κB activity in the cortex of rat.

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posted on 2013-02-20, 03:14 authored by Yanlin Chen, Xuemei Wu, Shanshan Yu, Xuemei Lin, Jingxian Wu, Lan Li, Jing Zhao, Yong Zhao

(A) Nuclear proteins of brain samples of three groups were assayed for NF-κB binding activity by EMSA 24 hours after focal cerebral I/R. Lane 1, positive control; Lane 2, sham group; Lane 3, vehicle+I/R group; Lane 4, TSA+I/R group. (B) Quantification of NF-κB binding activity was performed by densitometric analysis. Bar graph showing the relative density of the EMSA autoradiograph of the three groups compared to sham group. The figure indicates that cerebral NF-κB activity was significantly increased in vehicle+I/R group (#P<0.05), which was significantly lower in the TSA treatment group than in the vehicle+I/R group (*P<0.05). Data represents mean ± S.E.M. (n = 5 per group).

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