MyD88 signaling in IEC is not required for protection against C. rodentium infection.
Myd88 −/−, Myd88 flox and IEC-Myd88 −/− mice were infected for 6 days with C. rodentium. Infected IEC-Myd88 −/− mice carried similar (A) pathogen burdens, (B) levels of serum FD4, and (C–D) similar mucosal damage as Myd88 flox mice. Moreover all of these readouts are significantly greater in Myd88 −/− mice, as compared to Myd88 flox and IEC-Myd88 −/− mice. Pathogen counts represent mucosal associated bacteria. Results are pooled from 2 independent infections with n = 3–4 mice per group. Error bars = SEM, (Student t test *P<0.05, ** P<0.01). Images were taken at 200× magnification.