Motion parameters and percentage of damaged boar spermatozoa during BTS preservation at 16°C in the presence of 250 µg/mL gentamicin (standard), 4 µM and 8 µM c-WFW, 2 µM and 4 µM c-WWW, and 1 µM and 2 µM MK5E (n = 9).
The percentage, the velocity (VAP), and the linearity (VSL/VCL) of progressive motile spermatozoa after 12 h and 96 h storage are shown (A, C, E). The percentage and the velocity (VAP) of progressive motile spermatozoa after 48 h storage are shown in a thermoresistance test (TRT, incubation at 38°C) for 30 and 300 min (B, D). The percentage of damaged spermatozoa was determined after 12 h, 48 h, and 96 h storage by staining with propidium iodide and/or markers for the acrosomal membrane and contents (F). Significant difference (see text for the exact P-values) compared to standard after 12 h (▪), 48 h (#), 96 h (▴) storage, and after TRT 30 min (○) and TRT 300 min (•) incubation at 38°C.