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Morphology of differentiated neuroblastoma cells silenced for hStau1 gene.

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posted on 25.11.2014, 03:06 authored by Joan Peredo, Patricia Villacé, Juan Ortín, Susana de Lucas

Cultures of SH-SY5Y neuroblastoma cells previously transduced with control (Ctrl) or hStau1-specific (siStau) silencing lentiviruses were treated for 72 h with doxicyclin and then induced for differentiation as described in Materials and Methods. At day 7 post-differentiation, the cultures were fixed and immunostained with an antibody specific for ßIII-tubulin (blue). Phalloidin (green) were used to detect actin filaments. Red colour corresponds to the RFP signal. Dendrites of 50 cells chosen at random were measured for each sample. (A) Representative images of not silenced and silenced differentiated SH-SY5Y cells. (B) The graph shows the total dendrite length for control or silenced cells. (C) The graph presents the dendritic length per dendritic order.