Morphological features of the manchette during rat spermatogenesis.
(A,B) Electron microscopy. (A) This electron micrograph shows the absence of the manchette at the start of acrosome formation in rat spermatogenesis. N: nucleus, PA: proacrosome (B) This electron micrograph shows the presence of the manchette (arrows) during nuclear shaping in rat spermatogenesis. N: nucleus (C,D) Immunofluorescent staining. These immunofluorescent light micrographs show the localization of tubulin (green staining) and KIFC1 (red staining) in rat spermatids. The manchette appears to surround the nucleus in a skirt-shaped manner.