Molecular identification of causative CL species.
Restriction fragment length polymorphism (RFLP) analysis of the amplified internal transcribed spacer 1 region (ITS1) digested with restriction enzyme HaeIII and analysed by electrophoresis on 2.5% agarose gels. Three reference strains were used for comparison; Lane 1 = L. major: MHOM/PS/01/ISL659, Lane 2 = L. tropica: MHOM/PS/02/63JnF21 and Lane 3 = L. infantum MHOM/TN/1980/IPT1. 1 kb = molecular size marker. All other lanes show digested PCR product from clinical materials; lanes 4–7 = L. tropica cases from Al Jabal Al Gharbi, Misrata and Tarhuna districts; lanes 8–11 = L.major cases from Tripoli, Sirt, Misrata, Al Murqub.