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Molecular characterization of Ascaris by PCR-RFLP.

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posted on 11.04.2013, 00:00 by Serena Cavallero, Viliam Snabel, Francesca Pacella, Vitantonio Perrone, Stefano D'Amelio

A representative gel displaying the RFLP profiles following the digestion of ITS amplicons with restriction endonuclease HaeIII. Genotype As: Ascaris suum banding pattern (lanes 1–3,5,7–9,11); genotype Al : A. lumbricoides banding pattern (lanes 4,10,12,13); genotype H: hybrid/heterozygote banding pattern (lane 6); R (right-hand lane): 100bp reference ladder.