Figure_2.tif (864.88 kB)

Modulation of viral infection and production of R5 and X4 HIV strains by visfatin.

Download (0 kB)
figure
posted on 20.02.2013, 06:47 by Rafael Van den Bergh, Sébastien Morin, Hans Jürgen Sass, Stephan Grzesiek, Marc Vekemans, Eric Florence, Huyen Thanh Thi Tran, Rosina Gabriel Imiru, Leo Heyndrickx, Guido Vanham, Patrick De Baetselier, Geert Raes

Infection of MDM and resting PBMC (pre-)treated with recombinant visfatin (200 ng/ml) by R5 HIVBaL, HIV968-2 and HIV968-3 and X4 HIVIIIB, HIV968-1 and HIV968-4: A-B) TCID50 values were determined using the method of Reed & Muench[24], based on p24 measurement in culture supernatants of respectively MDM and PBMC. Infection in visfatin treated cells is expressed as a percentage of infection in untreated control cells from the same donor (a): an infection could not be established in absence of visfatin treatment; results for six independent donors are shown; C-D) viral production, as quantified by p24 secretion, 14 days after respectively MDM and PBMC infection with the different strains at 0.1 MOI; means ± SEM of six independent donors are shown; E) time of addition: MT-2 cells were infected with R5 HIVBaL and HIV968-2 and were treated with visfatin (200 ng/ml) after the indicated times; p24 production was assessed after 4 days of infection; F) viral production, as quantified by p24 secretion, 14 days after MDM infection with R5 HIVBaL, HIV968-2 and HIV968-3 at 0.1 MOI, in the presence of different doses of visfatin (dose-response curve). NT: untreated control.

History

Licence

Exports

Logo branding

Licence

Exports