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Modification of the flhDC master operon.

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posted on 13.03.2013, 09:32 by Thomas Heel, Georg F. Vogel, Andrea Lammirato, Rainer Schneider, Bernhard Auer

The expression strain HMS174(DE3) was used for modifications of the bacterial genome. Upstream regulator elements of the master operon flhDC were deleted and replaced by the artificial araBAD promoter resulting in the HMS174(DE3)ΔinsAB araBAD-flhDC strain. Activator elements are depicted in green, whereas repressor elements are depicted in red [6], [40].