Model Illustrating Target Sequence Dependency for Cas9 Target Binding and Cleavage.
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posted on 2014-10-02, 02:54 authored by Regina Cencic, Hisashi Miura, Abba Malina, Francis Robert, Sylvain Ethier, T. Martin Schmeing, Josée Dostie, Jerry PelletierFollowing PAM sampling by the Cas9:sgRNA complex, only PAM-proximal sequences are required for Cas9:sgRNA:target nucleation and are sufficient for complex formation [11]. Our study indicates that PAM-distal complementarity with the sgRNA (and a minimum sgRNA guide length of 17 nucleotides) is required to engage Cas9 target cleavage.
