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Mitochondrial localization of human SelO.

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posted on 21.04.2014 by Seong-Jeong Han, Byung Cheon Lee, Sun Hee Yim, Vadim N. Gladyshev, Seung-Rock Lee

(A) Schematics of recombinant SelO. It contains the mitochondrial leader sequence (MLS) at the N-terminus and the green fluorescent protein (GFP) at the C-terminus. Sec was changed to Cys. (B) Western blotting of SelO, TR2, tubulin, and TR1 in two separate fractions, cytosolic and mitochondrial, isolated from HEK293T cells. (C) Mitochondrial localization of recombinant SelO. The EGFP signal is shown in green and Mito-Tracker in red.

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