MiR-210 expression is increased in CRC tissues and associated with a poor prognosis.
(A) MiR-210 expression was tested using qRT-PCR in 193 pairs of human CRC tissues (CRC) and adjacent non-tumorous tissues (NT), and its expression was normalized to the level of U6 small nuclear RNA (U6) expression in each sample. (B) Fold changes in miR-210 expression in each paired sample. The data are represented as a log2-fold change (cancer/normal), which was defined as >1 (overexpression) and <−1 (underexpression); the remaining fold changes were defined as unchanged. (C) The overexpression of miR-210 was found in 58% (111 of 193) of the CRC tissues compared to the adjacent non-tumorous tissues. (D) HIF1α mRNA expression was detected in CRC tissues (CRC) and adjacent non-tumorous tissues (NT), and its expression was normalized to the level of β-actin expression in each sample. (E) The correlation between miR-210 expression and HIF1α mRNA levels in CRC tissues was analyzed using Pearson's correlation analysis. (F) Kaplan-Meier overall survival curves according to the level of miR-210 expression. The patients with high miR-210 expression had a significantly poorer 5-year overall survival rate than those with low miR-210 expression (P<0.001). The median miR-210 expression level in the tumor samples was chosen as the cut-off point.