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Metformin delayed repair of IR-induced DNA damage in p53-deficient cells compared with p53 wild-type cells.

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posted on 2015-11-23, 14:48 authored by Youn Kyoung Jeong, Mi-Sook Kim, Ji Young Lee, Eun Ho Kim, Hunjoo Ha

HCT116 p53+/+ and p53-/- cells were pretreated with 2.5 mM metformin for 24 h, and then irradiated with 6 Gy. (A) Immunofluorescence staining at 6 and 24 h after IR, showed γ-H2AX, a marker for DNA damage; Rad51, a marker of DNA repair; and nuclear DNA stained with DAPI using image analysis in three (green/red/blue) fluorescence channels. (B) For quantitative analysis, γ-H2AX or Rad51 foci-positive cells were counted in at least 100 cells from randomly captured images. Values are mean ± S.E.M, *p < 0.001. IR, ionizing radiation; γ-H2AX, γ-H2A histone family, member X.