Figure_2.tif (192.01 kB)
Metal coordination mutations eliminate the ability of Mlh1-Pms1 to nick closed circular DNA.
figure
posted on 2013-10-31, 03:19 authored by Catherine E. Smith, Marc L. Mendillo, Nikki Bowen, Hans Hombauer, Christopher S. Campbell, Arshad Desai, Christopher D. Putnam, Richard D. KolodnerEndonuclease reactions to nick closed circular DNA were performed with Mlh1-Pms1 alone or with Mlh1-Pms1, PCNA and RFC-Δ1N. A. Percentage of wild-type cleaved product formed by wild-type Mlh1-Pms1 and mutant Mlh1-Pms1 complexes containing the indicated Pms1 amino acid changes. B. Percentage of wild-type cleaved product formed by wild-type Mlh1-Pms1 and mutant Mlh1-Pms1 complexes containing the indicated Mlh1 amino acid changes. 100% cleavage of the 100 ng of pRS425 in the assay is 2.2 fmoles of cleavage events.
