Metagenomic exploration of the resistome from human and environmental sources.

Relative distribution of reads assigned to three functional subsystems among 27 metagenomes (based on MG-RAST annotation, E-value  =  10⁻⁵). Data are normalized by the total annotated sequences and are expressed as a percentage. The horizontal line in each box plot represents the mean of the relative distribution in each of the five environments (oceans, soils, freshwater, human feces, and WWTPs). The 27 metagenomes used for the analysis are available at http://metagenomics.anl.gov[13]. Accession numbers for oceans: 4441573.3, 4441574.3, 4441576.3, 4441577.3, 4441591.3, and 4443729.3; soils: 4441091.3, 4445990.3, 4445993.3, 4445994.3, 4445996.3, and 4446153.3; freshwater (rivers): 4511251.3, 4511252.3, 4511254.3, 4511255.3, 4511256.3, and 4511257.3; human feces: 4440595.4, 4440460.5, 4440611.3, 4440614.3, 4440825.3, and 4461119.3; and WWTPs: 4455295.3, 4463936.3, and 4467420.3.