Mesenchymal stem cells enhance neurite outgrowth from the human SH-SY5Y neuronal cell line.

(A) Immunocytochemical staining for βIII tubulin (Alexa Fluor 488) visualized neurite outgrowth (highlighted with arrows) from SH-SY5Y cells following 24 h co-culture with medium only controls (α-MEM and diff α-MEM) and undifferentiated MSC (uMSC) and differentiated MSC (dMSC) from young and old donors seeded in tissue culture inserts above the neurons. (B) Quantitative analysis of total neurite outgrowth in the co-cultures. ***P<0.001 significantly different to respective media only controls; *P<0.05 significantly different young dMSC versus old dMSC; ns not significantly different to respective media only controls.