Mass spectrometric IgG1 Fc-glycosylation profiles of A) IVIg and B) IVIg-SA (+).
A) IVIg and B) IVIg-SA (+) were subjected to tryptic cleavage and analyzed by nanoLC-ESI-ion trap-mass spectrometry. Sum mass spectra of the elution range of the IgG1 Fc-glycopeptides are shown. Glycopeptides were registered as proton adducts ([M+4H]4+). All the displayed glycopeptides have 1 missed tryptic cleavage site and share the peptide moiety T289KPREEQFNSTFR301 carrying a glycan at N297. *, contaminant or irrelevant peak.