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Macroscopic examination and engraftment of SPIO-labeled cells in non-infarcted heart.

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posted on 27.03.2015, 04:15 by Rebeca Blázquez, Francisco Miguel Sánchez-Margallo, Verónica Crisóstomo, Claudia Báez, Juan Maestre, Mónica García-Lindo, Alejandra Usón, Verónica Álvarez, Javier G. Casado

The SPIO-labeled pBM-MSCs were intrapericardially injected. At 7 days, heart samples were sliced into 1–3 cm short-axis sections and were then photographed (A). The heart slices were fixed in 4% formaldehyde. For the detection of SPIO–labeled cells, tissue sections were incubated with 8% hydrochloric acid and 4% potassium ferrocyanide and then with eosin. The blue color indicates the presence of SPIO within the tissue (B). Finally, for histological examination, tissue sections were paraffin-embedded and Prussian-blue/eosin staining demonstrated a preferential distribution of SPIO–labeled cells in the left ventricular myocardium (C). Scale bar: 100μm. Black arrows indicate the presence of SPIO-labeled cells evidenced by the potassium ferrocyanide staining.