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M.fermentans diminished the CPT inhibition effect on the DNA relaxation activity of Topo I.

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posted on 27.08.2013 by Reuven Afriat, Shulamith Horowitz, Esther Priel

MCF7 cells were infected with M. fermentans (M.f) for 6 hrs followed by CPT (30 µM) treatments for additional 1.5 hrs. Total nuclear protein (12.5 ng) was added to a specific reaction mixture for Topo I. Reaction products were analyzed by agarose gel electrophoresis. (A) A representative picture n = 5, of Topo I DNA-relaxation activity. (B) Quantification analysis of Topo I activity. (C) Topo I protein level examined by Western blot analysis. Symbols: R and S are the relaxed and supercoiled form of the pUC19 DNA, respectively, Topo- no protein added to the reaction mixture t-test: *p<0.05, **p<0.01, ***p<0.005.