M.fermentans diminished the CPT inhibition effect on the DNA relaxation activity of Topo I.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
MCF7 cells were infected with M. fermentans (M.f) for 6 hrs followed by CPT (30 µM) treatments for additional 1.5 hrs. Total nuclear protein (12.5 ng) was added to a specific reaction mixture for Topo I. Reaction products were analyzed by agarose gel electrophoresis. (A) A representative picture n = 5, of Topo I DNA-relaxation activity. (B) Quantification analysis of Topo I activity. (C) Topo I protein level examined by Western blot analysis. Symbols: R and S are the relaxed and supercoiled form of the pUC19 DNA, respectively, Topo- no protein added to the reaction mixture t-test: *p<0.05, **p<0.01, ***p<0.005.