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MS/MS identification of the disulfide connectivity for MCh-1.

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posted on 08.10.2013 by Wen-Jun He, Lai Yue Chan, Richard J. Clark, Jun Tang, Guang-Zhi Zeng, Octavio L. Franco, Cinzia Cantacessi, David J. Craik, Norelle L. Daly, Ning-Hua Tan

(A) ESI-TOF MS spectrum of the chymotrypsin digest of alkylated IIa isolated from the selective reduction experiments of the native MCh-1. (B) ESI-TOF MS spectrum of chymotrypsin digest of alkylated Ia isolated from the oxidative refolding of the fully reduced MCh-1. The phenylalanine residues that define the digestion positions are indicated with asterisks.

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