MS/MS identification of the disulfide connectivity for MCh-1.
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(A) ESI-TOF MS spectrum of the chymotrypsin digest of alkylated IIa isolated from the selective reduction experiments of the native MCh-1. (B) ESI-TOF MS spectrum of chymotrypsin digest of alkylated Ia isolated from the oxidative refolding of the fully reduced MCh-1. The phenylalanine residues that define the digestion positions are indicated with asterisks.