MMPs cleave MBP, BG21 and J37.

A, Gel-electrophoresis of the digest samples. MBP, BG21 and J37 were incubated alone for 60 min at 37°C or co-incubated with the individual MMPs at the indicated enzyme-substrate molar ratio. Where indicated, GM6001 was added to the reactions to block MMPs. B, The MBP isoforms are inefficiently cleaved by certain MMPs. MBP, BG21 and J37 were each co-incubated for 60 min at 37°C with the indicated MMPs at a 1∶100 enzyme-substrate ratio.