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MK does not activate NFκB signaling in 3T3-L1 adipocytes.

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posted on 07.02.2014, 07:57 by Nengguang Fan, Haiyan Sun, Yifei Wang, Lijuan Zhang, Zhenhua Xia, Liang Peng, Yanqiang Hou, Weiqin Shen, Rui Liu, Yongde Peng

A. Differentiated 3T3-L1 adipocytes were treated with recombinant MK for the indicated time periods. Phosphorylation of p65/NFκB (Ser536) was assessed by western blot analysis and representative results are shown. B. Intensity of bands was quantified by densitometry. Phosphorylated p65/NFκB levels were normalized to GAPDH and expressed as fold of controls. C, D. Differentiated 3T3-L1 adipocytes were treated with increasing dose of recombinant MK for 16 h. IL-6 (C) and MCP-1 (D) mRNA expression were evaluated by quantitative RT-PCR. Relative gene expression was expressed as fold of controls. Data are mean ± SE; n = 3. *P<0.05 versus controls.

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