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MIF treatment reduces TGF-β in monocytes and ameliorates the profibrogenic effects of Vpu expressing monocytes.

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posted on 13.02.2014 by Paresh Patel, Nabab Khan, Manjusha Rani, Deepti Gupta, Shahid Jameel

(A) Quantitative RT-PCR estimation of TGF-β mRNA levels in U937-GFP and U937-VpuGFP cells treated with MIF. The expression levels are shown as fold changes normalized to the untreated controls. (B) Quantitation of TGF-β in the culture supernatants of the U937-VpuGFP and U937-GFP cultures treated with MIF or untreated controls. (C) LX2 cells were cocultured with either U937-GFP or U937-VpuGFP cells together with MIF as described in Methods. Quantitative RT-PCR was then carried out to estimate the expression levels of mRNAs for COL-1, MMP2, PCT-III and TGF-β in LX2 cells. All expression levels are shown as fold changes normalized to the U937-GFP control. (D) LX2 cells were cocultured with U1-scrambled cells together with MIF, and quantitative RT-PCR was used to estimate the expression levels of COL-1 and αSMA-1 mRNAs; the values are expressed as fold changes normalized to the untreated culture condition. The data are representative of three biological replicates. Error bars represent mean ± SD; *p<0.05.

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