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MICA/B+ cells in the lamina propria.

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posted on 2013-09-13, 02:30 authored by Yessica L. Allegretti, Constanza Bondar, Luciana Guzman, Eduardo Cueto Rua, Nestor Chopita, Mercedes Fuertes, Norberto W. Zwirner, Fernando G. Chirdo

A.- Immunofluorescent confocal microscopic analysis was performed in paraffin embedded sections from tissues with severe enteropathy (i, iii, iv, v, vi, vii, viii) and mild enteropathy (ii). Sections were stained as follows: MICA/B (red), Nuclei (blue). i. CD3+ cells (green). ii and iii. CD7+ cells (green).. iv. CD20+ (green). v. CD138+ cells (green). vi. HAM-56+ cells (green). vii. CD11c+ cells (green). viii. IC antibody (all cell lineage markers in green). (scan zoom 0.7, magnification 100×). B.- Expression of MICA/B in CD7+ cells in sections of small intestine samples of 6 healthy controls, 8 mild enteropathy samples and 4 severe enteropathy samples. Percentage of MICA/B+ cells in the CD7+ population (left panel) and total number of lamina propria CD7+ cells per unit of m.m. (right panel) were plotted. * p≤0,05; (Non parametric Kruskal wallis test followed by the Dunns multiple-comparison posttest). C.- Expression of MICA/B in CD138+ cells in sections of small intestine samples of 13 healthy controls, 7 mild enteropathy and 5 severe enteropathy. Percentage of MICA/B+ cells on the CD138+ population (left panel) and total number of lamina propria CD138+ cells per unit of m.m (right panel). ** p≤0.01, (Non parametric Kruskal wallis test followed by the Dunns multiple-comparison posttest).

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