MHC-I cell surface-to-lysosome trafficking in DCs is impaired by mutation in the cytoplasmic tyrosine residue.
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(A to D) Splenic DCs isolated from KbWT, and Δ7 and ΔY transgenic mice were mounted on coverslips, labeled with FITC-conjugated H-2Kb-specific mAb, washed, and incubated at 37°C for the indicated times. DCs were then fixed, permeabilized, and counterstained for LAMP-1. Yellow color indicates co-localization of surface-derived H-2Kb (green) with LAMP-1 (red). Data are representative of at least 3 images captured from 2 independent experiments.