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MCP-1/CCR2 axis in hypothalamus and peripheral blood, and effects of CCR2 blockade on the infiltration of bone marrow-derived microglia into the PVN and anxiety-like behavior induced by chronic PS.

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posted on 26.11.2013, 03:18 by Koji Ataka, Akihiro Asakawa, Kanna Nagaishi, Kaori Kaimoto, Atsushi Sawada, Yuko Hayakawa, Ryota Tatezawa, Akio Inui, Mineko Fujimiya

(A) mRNA expression of chemokines in hypothalamic tissue from chronic PS-loaded and sham-treated mice (n = 4). Data are expressed as mean ± sem. *P < 0.05 with two-tailed Student’s t-test. (B) Immunofluorescence staining with MCP-1 (red) and NeuN (pink) in PVN from chronic PS-loaded and sham-treated mice. Arrows indicate MCP-1+NeuN+ cells and arrow heads indicate MCP-1-NeuN+ cells. Scale bars: 20 µm. Data are expressed as mean ± sem (n = 7). *P < 0.05 with two-tailed Student’s t-test. (C) Immunofluorescence staining with MCP-1 (red) and GFAP (pink) in PVN from chronic PS-loaded and sham-treated mice. Scale bars: 20µm. Data are expressed as mean ± sem (n = 4−6). *P < 0.05 with two-tailed Student’s t-test. (D) Frequency of GFP+CCR2+ cells in the peripheral blood obtained by FACS (n = 4−6). Data are expressed as mean ± sem. *P < 0.05 with two-tailed Student’s t-test. (E) The number of CCR2+ cells in the hypothalamus obtained by FACS (n = 4). Total events in FACS were 20000. Data are expressed as mean ± sem. *P < 0.05 with ANOVA followed by Tukey’s multiple comparison. (F) Effects of a CCR2 antagonist, RS102895, on infiltration of bone marrow-derived microglia in PVN. PVN is shown by dotted line. Scale bars: 50 µm. Number of GFP-positive cells in a section including PVN is shown. (G) Effect of RS102895 on the anxiety-like behavior induced by chronic PS. Data are expressed as mean ± sem. *P < 0.05 with ANOVA followed by Tukey’s multiple comparison (n = 4).

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