Lyve-1^wt/Cre;Vegfr2^flox/flox mice exhibit a deficiency of dermal lymphatic vessels.

(A,B) Whole-mount immunofluorescence staining of ear skin from Vegfr2^flox/flox and Lyve-1^wt/Cre;Vegfr2^flox/flox mice for Lyve-1. (C) Quantitative analysis showing that there are significantly fewer lymphatic branch points in the ear skin of Lyve-1^wt/Cre;Vegfr2^flox/flox mice (6.042±0.198, n = 6) than Vegr2^flox/flox mice (11.29±0.940, n = 6). (D) Lymphatic vessel diameter is not significantly different between Lyve-1^wt/Cre;Vegfr2^flox/flox (52.65 µm±1.303, n = 6) and Vegfr2^flox/flox mice (49.05 µm±1.675, n = 6). *** indicates P || 0.001.