Lower CD4 T-cell count in PTB individuals as compared to healthy controls.

A. Representative dot plot showing gating strategy for immunophenotyping of the three subpopulations of CD4⁺ T-cells based on CD25 expression as: CD25^high, CD25^intermediate and CD25^low/negative. B. Representative overlay histogram showing intracellular FoxP3 expression in different CD4⁺ T-cell subpopulations with isotype control (upper right). C. Bar diagram showing mean±SEM of CD4 counts of each studied group. HC: Healthy control; PTB: pulmonary tuberculosis; HIV >500: HIV-1 infected individuals with CD4 count above 500 cells/µl; HIV 500>X>250: HIV-1 infected individuals with CD4 count between 500 and 250 cells/µl; HIV <250: HIV-1 infected individuals with CD4 count less than 250 cells/µl. Significant differences are represented as the p value <0.01 (**), NS: (non-significant).