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Loss of one polarity gene and cooperation with pro-tumorigenic cytokines.

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posted on 18.04.2012 by Samit Chatterjee, Laurie Seifried, Michael E. Feigin, Don L. Gibbons, Claudio Scuoppo, Wei Lin, Zain H. Rizvi, Evan Lind, Dilan Dissanayake, Jonathan Kurie, Pam Ohashi, Senthil K. Muthuswamy

(A) Phase images showing induction of invasion in 4-day old acini of parental MCF10A cells or cells expressing shRNAi for indicated polarity genes growing in M/Col-I matrix and treated with 1∶6 diluted supernatant from CpG-treated dendritic cells (DC Sup.), or pro-inflammatory cytokines TNF-α (2 ng/ml) and IL-6 (25 ng/ml) (refer to SI Fig. S3A). Arrows indicate region of the image magnified in the inset. Scale bars, 100 µm (B) Quantification of invasion of DC Sup-treated acini plotted as mean ± S.E.M. from at least three independent experiments. (C–D) Quantification of invasion in MCF10A acini after treatment with recombinant TNF-α (C) or IL-6 (D). Also refer to SI Fig. S3B,C. *, p<0.05, **, p<0.005. p values are based on an unpaired t-test comparing cytokine-treated to the untreated values for the same knockdown.

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