Loss of membrane asymmetry after IITT+F treatment.
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Non-differentiated (St-T1, KdS1) and decidualized (dSt-T1, dKdS1) ESCs were treatmed with IITT+F and loss of membrane asymmetry was visualized with Annexin V FITC staining (green), n = 3; (A)–(D) untreated controls: (A) St-T1, (B) KdS1, (C) dSt-T1, (D) dKdS1. (E)-(H) IITT+F treated: (E) St-T1, (F) KdS1, (G) dSt-T1, (H) dKdS1; blue nuclei are stained with Hoechst 33342. Scale bars indicate 100μm.