Loss of hDlg1 enhances resistance to anoikis.

Panel A. Cells were plated on tissue culture dishes that were either untreated, or coated with poly-HEMA to block cell attachment. After 24 h the cells were harvested and stained with Propidium Iodide, and the cell cycle distribution of a representative clone was ascertained by flow cytometry. Panel B shows the mean percentage of cells entering apoptosis (as determined by sub-G1 DNA content) from at least three separate experiments with three independent cell lines together with the standard deviations. Panel C. Cells were processed as in Panel A, but were stained with Trypan Blue. The results show the mean percentage of dead and dying cells, as determined by Trypan Blue uptake from at least three independent experiments on at least three independent cell lines together with the standard deviations. Panel D. Cells were treated with etoposide or exposed to UV C. After 12 h incubation the cells were stained with Propidium Iodide and the sub-genomic DNA content determined by flow cytometry, with the results shown being from one representative clone of each line.