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Localization of phospho-PKR and total-PKR in HCT116 p53+/+ and HCT116 p53−/− cells.

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posted on 2013-02-20, 15:28 authored by María Angel García, Esther Carrasco, Margarita Aguilera, Pablo Alvarez, Carmen Rivas, Joaquin María Campos, Jose Carlos Prados, Miguel Angel Calleja, Mariano Esteban, Juan Antonio Marchal, Antonia Aránega

(A) HCT116 p53+/+ cells and (B) HCT116 p53−/− cells were mock treated or treated with 5 µM of 5-FU. At the indicated times cells were fixed and processed for immunofluorescence analysis by confocal microscopy as described in Material and Methods using antibodies against phospho-PKR (green) and anti- whole PKR (green) and DAPI for staining nuclei (blue).