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Localization of phospho-PKR and total-PKR in HCT116 p53+/+ and HCT116 p53−/− cells.

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posted on 20.02.2013, 15:28 by María Angel García, Esther Carrasco, Margarita Aguilera, Pablo Alvarez, Carmen Rivas, Joaquin María Campos, Jose Carlos Prados, Miguel Angel Calleja, Mariano Esteban, Juan Antonio Marchal, Antonia Aránega

(A) HCT116 p53+/+ cells and (B) HCT116 p53−/− cells were mock treated or treated with 5 µM of 5-FU. At the indicated times cells were fixed and processed for immunofluorescence analysis by confocal microscopy as described in Material and Methods using antibodies against phospho-PKR (green) and anti- whole PKR (green) and DAPI for staining nuclei (blue).