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Lin28 expression in breast cancer cell lines and the transfection efficiencies of the Lin28-siRNAs, Lin28-expressing plasmid and Lin28-mutant plasmid.

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posted on 2013-12-11, 04:08 authored by Yujie Liu, Haiyan Li, Juan Feng, Xiuying Cui, Wei Huang, Yudong Li, Fengxi Su, Qiang Liu, Jiujun Zhu, Xiaobin Lv, Jianing Chen, Di Huang, Fengyan Yu

A. Differential expression of Lin28 was analyzed by Western blotting in multiple breast cell lines, including MCF-7, SK-3rd, MCF-10A, MDA-MB-231 and BT-474. B. Western blotting showed that transient transfection of either of the Lin28-siRNAs in MDA-MB-231 cells, compared with GFP-siRNA transfection, led to a reduction in the expression of Lin28. C and D. Lin28 expression was determined by PCR (C) and Western blotting (D) in MCF-7 cells that were stably transfected with pc-Lin28-1, pc-Lin28-2 (C and D) or Lin28-mut (D). GAPDH was used as a loading control.

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