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Limited proteolysis of GmaR and MogR with chymotrypsin indicates a temperature-dependent conformational change in GmaR, but not MogR.

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posted on 2011-08-04, 00:02 authored by Heather D. Kamp, Darren E. Higgins

Purified GmaR-His6 or MogR-His6 was incubated with chymotrypsin (5000∶1) for 60 min at either RT or 37°C. Reactions were stopped at the sample times indicated by removing 5 µg of protein and mixing with 2X loading buffer. Samples were run on an SDS-PAGE gel and stained with Coomassie stain.