LbGAP Displays GAP Activity In Vitro

Absorbance at 650 nm is correlated with the amount of Pi released from GTP-bound human Rac1, Cdc42, RhoA or Ras, either in the presence of LbGAP or p50RhoGAP as a control or in the absence of any GAP. Grey bars: GAP activity in the presence of LbGAP. Open bars: GAP activity in the presence of p50RhoGAP. Black bars: GAP activity in the absence of GAP. For each value, bars represent the standard error of three measurements. Different letters above the bars designate significantly different GAP activity results (p <0.001).