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LbGAP Displays GAP Activity In Vitro

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posted on 2007-12-28, 00:00 authored by Dominique Colinet, Antonin Schmitz, Delphine Depoix, Didier Crochard, Marylène Poirié

Absorbance at 650 nm is correlated with the amount of Pi released from GTP-bound human Rac1, Cdc42, RhoA or Ras, either in the presence of LbGAP or p50RhoGAP as a control or in the absence of any GAP. Grey bars: GAP activity in the presence of LbGAP. Open bars: GAP activity in the presence of p50RhoGAP. Black bars: GAP activity in the absence of GAP. For each value, bars represent the standard error of three measurements. Different letters above the bars designate significantly different GAP activity results (p <0.001).