Laser scanning microscopy showing comparison of vimentin and adipophilin antibodies in AIM-stimulated compared to AIM-stimulated plus shortly OA-treated human preadipocytes.
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(a) With short AIM stimulation for one day many tiny LDs positively stained for adipophilin can still be seen within the dense vimentin meshwork. (a) Additional short OA treatment for 3 hours immediately leads to exogenously derived, larger adipophilin positive droplets. Arrowheads mark two huge, vimentin surrounded cage-like structures. These are probably perilipin containing droplets (cp. Fig. 7d,d) which have not yet been amalgamated by the new exogenously generated adipophilin droplets. DAPI (blue). Bars: 20 µm.