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Lack of sortilin reduces APP distribution in lysosome and increases APP distribution in lipid rafts.

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posted on 21.05.2013, 01:30 by Miao Yang, Balaji Virassamy, Swarna Lekha Vijayaraj, Yoon Lim, Khalil Saadipour, Yan-Jiang Wang, Yan-Chuang Han, Jin-Hua Zhong, Carlos R. Morales, Xin-Fu Zhou

Colocalization of APP with cell organelles in cortical neurons. Wild type (WT) and sortilin knockout (KO) mouse cortical neurons were immunostained for APP with mouse anti-APP-N’ (22c11) and followed by staining with Cy3 conjugated secondary antibodies (red) and cell organelles: Golgi, Early endosome, late endosome and lysosome immunostained with Giantin, EEA1, anti-mannose 6 phosphate receptor (for late endosome) and Lamp1, followed by staining with Alexa 488 conjugated secondary antibodies (green). Cell nuclei are stained by DAPI (blue). The colocalization is indicated in merged panels (yellow). Snapshots of colocalization are shown on the right of each panel. The percentage of colocalization (Col.) is plotted and is represented as mean± SEM (n = 20). The colocalization of APP with cell organelles is compared between WT and KO neurons. The star (*) indicates p<0.01. Scale bar 10 µm.

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