Lack of Hic-5 has no influence on platelet adhesion to collagen, spreading on fibrinogen and platelet integrin outside-in signaling in vitro.
(A, B) Adhesion (A) and thrombus formation (B) of platelets on collagen was assessed in a flow adhesion assay at a wall shear rate of 1000 s-1. Scale bar, 25 μm. (C, D) Washed platelets of WT and Hic-5-null mice were allowed to spread on fibrinogen for up to 30 minutes after stimulation with 0.01 U mL-1 thrombin. (C) Representative images and (D) statistical evaluation of the percentage of spread platelets at different spreading stages are shown. Scale bar, 5 μm. (E, F) Clot formation in platelet-rich plasma was induced by the addition of thrombin (5 U mL-1) and 20 mmol L-1 CaCl2 and clot retraction was monitored over time (E). (F) Residual volume of serum after clot retraction was measured.