LRIG2 or LRIG2ecto overexpression inhibits the spontaneous apoptosis of glioblastoma cells in vitro.
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(A) Cells were cultured in DMEM with 10% FBS for 48 h, stained by Annexin V/PI and subjected to flow cytometry analysis. Early apoptotic populations were in the lower-right quadrant and late apoptotic cells were in the upper-right quadrant. Representative images were shown in the left panel and percentages of early and late apoptotic cells were expressed as the mean±SD of three independent experiments, shown in the right panel (*P<0.05 vs con). (B) Cells were maintained in complete medium for 48 h, stained with JC-1 and analyzed by flow cytometry. Representative flow cytometric images of mitochondrial membrane potential were shown. The shift of JC-1 fluorescence from red (FL2) to green (FL-1) indicates a collapse of the mitochondrial membrane potential. Change in mitochondrial membrane potential was determined by the ratio of JC-1 red fluorescence intensity to JC-1 green fluorescence intensity, shown in the right panel. Ratio was normalized to the control cells. (*P<0.05 vs con).