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LPS accelaerates SW480 colon cancer cell adhesion via Nox regulation of PI3K/Akt signalling.

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posted on 19.02.2013 by D. Peter O'Leary, Lavinia Bhatt, John F. Woolley, David R. Gough, Jiang H. Wang, Thomas G. Cotter, H. Paul Redmond

(a,b) LPS treatment resulted in a significant increase in SW480 cell adhesion to collagen I after 1 hour. This was inhibited using a recognised Nox inhibitor, DPI (2 µM), and a PI3K inhibitor, LY294002(5 µM). (c) Nox1 siRNA abrogates LPS induced ROS and Nox1 knockdown is confirmed by Western Blotting. (d) The increase in SW480 colon cancer cell adhesion was completely inhibited by Nox1 siRNA. * P<0.05. These data are representative of 3 independent experiments.

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