Ku0063794 and temsirolimus inhibited tumor growth in a xenograft model of RCC.
(A) The treatment with Ku0063794 or temsirolimus significantly inhibited tumor growth in Nu/Nu nude mice. Following subcutaneous injection of 786-O cells, mice (5 mice in each group) were treated from days 33–78. The error bars indicate the standard error of the mean. *p<0.05 when the Ku0063794 or temsirolimus group was compared with control. (B) 90 minutes after drug administration, both Ku0063794 and temsirolimus inhibited the mTORC1 pathway in vivo as indicated by decrease in S6P phosphorylation in the tumor tissues, while only Ku0063794 inhibited the mTORC2 pathway in vivo as indicated by decrease in Akt phosphorylation on Ser473. Each lane represents a tumor from a different mouse.