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Knockdown of eEF1Bγ inhibits TBSV RNA replication in N. benthamiana plants.

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posted on 15.12.2011, 00:56 by Zsuzsanna Sasvari, Lara Izotova, Terri Goss Kinzy, Peter D. Nagy

(A) Symptoms of TBSV infected plants 10 days after inoculation/19 days after agroinfiltration and the phenotype of the eEF1Bγ knockdown N. benthamiana plants 19 days after agroinfiltration with the VIGS vectors. VIGS was performed via agroinfiltration of Tobacco rattle virus (TRV) vectors carrying eEF1Bγ sequence or the TRV empty vector (as a control). (B) Reduced accumulation of TBSV RNA in the inoculated leaves of eEF1Bγ knockdown N. benthamiana plants 3 days post-inoculation, based on Northern blot analysis. Inoculation with TBSV gRNA was done by sap inoculation 9 days after silencing of eEF1Bγ expression. Ribosomal RNA is shown as a loading control at the bottom of the panel. (C) Reduced accumulation of TBSV RNA in the systemically-infected leaves of eEF1Bγ knockdown N. benthamiana plants 6 days post-inoculation. See further details in panel B.

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