Knockdown of α-enolase by siRNA reduces MPCs differentiation.
A. qRT-PCR to determine the relative mRNA quantity of α-enolase after siRNA transfection. B. Immunocytochemical staining for eMHC of MPCs transfected with α-enolase siRNA or control siRNA, on GM and DM at 24 and 48 hours. C. Quantification of the Fusion ratio (n° eMHC positive cells fused in a myotube >4 nuclei/total cells) or Myogenic Index. ** P<0.005. D. qRT-PCR to determine relative mRNA quantity of the genes for myogenin and eMHC. Ribosomal protein L32 mRNA levels were used for normalization. ** P<0.01, *** P<0.005. Results are the mean +/− SEM (error bars) of three different experiments.